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KMID : 0363620080290010047
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Journal of Korean Oriental Medicine
2008 Volume.29 No. 1 p.47 ~ p.59
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The Inhibitory Effect of Lycii Fructus on LPS-stimulated NF- Activation and iNOS Expression in RAW 264.7 Macrophages
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Kim Beum-Seuk
Song Yun-Kyung
Lim Hyung-Ho
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Abstract
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Objective: Anti-inflammatory effects of the extract of Lycii Fructus on lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 macrophage cells were investigated.
Method: In order to assess the cytotoxic effect of Lycii Fructus on the raw 264.7 macrophages 3-(4,5-
dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay was performed. Reverse transcription-polymerase
chain reaction(RT-PCR) analysis of the mRNA levels of tumor necrosis factor-α(TNF-α) and inducible nitric oxide
synthase(iNOS) was performed in order to provide an estimate of the relative level of expression of these genes. The
protein level of the inhibitor of nuclear factor-κB(IκB) and nuclear factor-κB(NF-κB) activity was investigated by Western blot assay. NO production was investigated by NO detection.
Result: Lycii Fructus suppressed NO production by inhibiting the LPS-induced expressions of iNOS and TNF- α
mRNA and iNOS protein in RAW 264.7 macrophage cells. Also, Lycii Fructus suppressed activation of NF-κB in the nucleus.
Conclusion: These results show that the extract of Lycii Fructus has anti-inflammatory effect probably by suppressing iNOS expressions through the down-regulation of NF-κB binding activity.
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KEYWORD
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Lycii Fructus, lipopolysaccharide, nitric oxide, inducible nitric oxide synthase, NF-κB
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